課程內容  Curriculum > Outline

各組課程  (生物工業組  微生物學組  生物化學組  營養科學組)  相關公告

  

mbc Course Outline

列出部份課程大綱,正在收集擴大中。Part of the course outlines, the list is in expanding.

Course ID

Course title

 

Status

B47 D0120

生物工業科技研究法

Special topics on bioindustrial research

 

B47 D0130

營養學研究法

Research tools in nutrition

 

 

     

B47 M0120

工物科技研究法二

Research methods in biotechnology

 

B47 M0150

工業微生物學

Industrial microbiology

 

B47 M0240

植物之二級代謝

Plant secondary metabolism

 

       

B47 U1230

生物分子動力學

Biomolecular kinetics

 

       

 大學部課程大綱

(To BST course outline)

B47 U1230

Biomolecular kinetics  生物 分子動力學

Instructors

楊健志、陸駿逸、陳佩燁

Outline

生物分子動力學係銜接生物化學,討論生物分子間交互作用的原理與研究方法。以蛋白質之結構與功能為起點,它們如何辨認、結合其他生物分子或催化反應進行,可以利用動力學及光譜學的方法加以研究,未知生理功能的蛋白質可利用酵母菌雙雜交法等分子生物學操作找出有交互作用的其他蛋白質。這些知識可做為進一步蛋白質設計或試管內演化的基礎。並延伸討論至 protein-ligand 及細胞內訊息傳導的研究。本課程預計與原研究所之酵素化學交替開授。

大 綱

 

Structure and Function Relationship of Proteins

Lecture 1

Introduction to protein structure

Copland 3; Fersht 1; Bourne 2

楊健志

Lecture 2

Determination of protein structure

Bourne 5; CPPS 17.3; Hammes 2

楊健志

Lecture 3

Molecular modeling

Tsai 14; Bourne 24, 25, 26

楊健志

Lecture 4

Molecular graphic and visualization

Bourne 7, 14, 15; Tsai 4

楊健志

 

Basic Enzyme Kinetics and Mathematic Aspects

Lecture 5

Rate of chemical reactions

Copland 2.5; Marangoni 1

陸駿逸

Lecture 6

Kinetics of single-substrate enzyme reactions

Copland 5; Tsai 7; Marangoni 3; Mikkelsen 2

陸駿逸

Lecture 7

Practical enzyme kinetics

Marangoni 14; Copland 7

楊健志

 

Protein Evolution and Protein-protein Interaction

Lecture 8

Directed evolution of protein

Mikkelsen 8; Brakmann 9

楊健志

Lecture 9

Detection of protein interactions

- phage display and yeast two-hybrid technologies

Brakmann 5, 7; Bourne 20

楊健志

 

Methods and Tools for Measuring Kinetics Parameters

Lecture 10

Electronic spectra

Hammes 3

陳佩燁

Lecture 11

Circular Dichroism

Hammes 4

陳佩燁

Lecture 12

Mass spectrometry

Hammes 8

陳佩燁

 

Protein-ligand Interaction and Signal Transduction

Lecture 13

Molecular recognition and Protein-ligand binding equilibria

Böhm 1, 2, 5; Copland 4; Bourne 22

楊健志

Lecture 14

Signal transduction by nuclear receptors and GPCR

Krauss 4, 5

楊健志

Textbook

Textbooks available as electronic pdf format from University library

 

Title/Year/Author

Publisher/ISBN

Böhm

Protein-ligand interactions from molecular recognition to drug design (2003) edited by H.-J. Bohm and G. Schneider.

Wiley-VCH 3527305211

Bourne

Structural Bioinformatics (Methods of Biochemical Analysis, V. 44) (2003) Philip E. Bourne, Helge Weissig

Wiley-Liss 0471201995

Brakmann

Directed molecular evolution of proteins : or how to improve enzymes for biocatalysis         (2002) Brakmann, Susanne.; Johnsson, Kai.

Wiley-VCH 3527304231

Copland

Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis (2000) Robert A. Copeland

Wiley-VCH 0471359297

CPPS

Current protocols in protein science

Wiley-Interscience

Hammes

Spectroscopy for the biological sciences (2005 ) Hammes, Gordon G

Wiley-Interscience

Krauss

Biochemistry of signal transduction and regulation (2000) Krauss, Gerhard.  .

Wiley-VCH 3527303782

Marangoni

Enzyme Kinetics : A Modern Approach (2002) Alejandro G. Marangoni

Wiley-Interscience 0471159859

Mikkelsen

Bioanalytical chemistry.(2004) Mikkelsen, Susan R., Cortón, Eduardo.

John Wiley & Sons 0471544477

Tsai

An Introduction to Computational Biochemistry (2003) C. Stan Tsai

Wiley-Liss

0471223840

 Other references

1. Alan Fersht.  (1998) Structure and Mechanism in Protein Science : A Guide to Enzyme Catalysis and Protein Folding. W. H. Freeman 0716732688     

2. Laszlo Patthy. (1999) Protein Evolution. Blackwell Science (UK) 0632047747

3. H. Gutfreund (1995) Kinetics for the Life Sciences. Cambridge University Press  052148586x   

 

Remarks  

B47 M0120

Research methods in biotechnology (II)  生物科技研究法

Instructors

李昆達 (Kung-Ta Lee), 黃健雄 (Jan-Hsiung Huang), 潘子明 (Tzu-Ming Pan), 劉俊民 (Chung Ming Liou)

Outline

1. Reading and discuss the articles in “Nature Biotechnology”

2. Methods for microbial cell cultivation and biotransformation (II)

3. The production and evaluation of fermentative functional foods

4. Microorganisms and Bioremediation 

 

大 綱

一、Nature Biotechnology 最新文章之導讀與討論

二、微生物培養與生物轉化方法 (下)

三、機能性發酵食品之生產與評估

四、微生物與環境復育

Textbook

No

Remarks

Lecture in English?  No

B47 D0120

Special topics on bioindustrial research  生物工業科技研究法

Instructors

潘子明 (Tzu-Ming Pan), 黃健雄 (Jan-Hsiung Huang), 劉俊民 (Chung Ming Liou), 李昆達 (Kung-Ta Lee)

Outline

Part 1: Searching of data and writing of manuscript

Part 2: Oral presentation and poster presentation

Part 3: Current status of microbial processing

Part 4: Biofunction of proteolytic Enzymes

Part 5: Trends in Plant Biotechnology

References

 1. Proteolytic enzymes: a practical approach. Edited by R. Beynon, J.S. Bond, Oxford University Press, 2001

 2. Proteolytic enzymes: aspartic and metallo peptidases. Edited by Alan J. Barrett, Academic Press, 1995

 3. Proteolytic enzymes: serine and cysteine peptidases. Edited by Alan J. Barrett, Academic Press, 1994

 4. Proteolytic enzymes: tools and target. Erwin E. Sterchi, Walter Stocker, Springer-Verlag Press, 1999

 5. Philipp, G., Murray, G. E., Willis, A. W. et. al. 1994. Methods for General and Molecular Bacteriology, American Society for Microbiology, Washington, D.C.

 6. Scheppler, J. A., Cassin, P. E., Gambier, R. M., 2000, Biotechnology Explorations, Applying the Fundamentals, American Society for Microbiology, Washington, D.C.

 7. K.Shinozaki, K.Yamaguchi-Shinozaki, and M. Seki: Curr.Opin.Plant Biol.,6,410-417 (2003)

Remarks

Lecture in English?  No

B47 M0150

Industrial microbiology  工業微生物學

Instructors

黃健雄 (Jan-Hsiung Huang), 劉俊民 (Chung Ming Liou), 李昆達 (Kung-Ta Lee)

Outline

 I. Introduction (Huang)

II. Fermentative production of bacterial metabolites (Huang)

     1. Primary metabolites

     2. Secondary metabolites

     3. Tertiary metabolites

III. Fermentative production of high molecular weight compounds, biopolymer and biodegradable plastics (Huang)

     1. Homopolymer and heteropolymer

     2. Characteristics of cultivation

     3. Structure-Property-Function relationship

     4. Application of modern biotechnology

IV. Fermentative production of low molecular weight compounds, flavor and fragrance ingredients (Huang)

     1. Characteristics of aroma

     2. Production methods

     3. Technical consideration

     4. Evaluation methods

V. Physiology and genetics of yeast (Liou)

     1. Cell wall and cell membrane

     2. Chromosome structure and function

     3. Mating of yeast                             

     4. Gene structure        

     5. Regulation of transcription

     6. GAL genes

     7. PHO Genes

VI. Heterologous protein expression in microorganism (Liou)

     1. Regulation of translation

     2. Tranfer RNA

     3. Ribosome structure and synthesis

     4. Initiatiion, elongation and termination of polypeptide synthesis

     5. Post-translational processing

     6. Couple of transcription and translation

     7. Expression vector of yeast

VII. Protein excretion in yeast (Liou)

     1. Protein folding stage

     2. Chaperone mechanism

     3. Quality control

     4. Secretion of proteins across the outer mbrane

VIII. Plant cell cultures (Lee)

     1. Products and application

     2. Process characteristics

     3. Molecular approach

     4. Future outlooks 

 

大 綱

一、課程介紹 (黃)

二、細菌代謝物之發酵生產 (黃)

     1. 初級代謝物

     2. 次級代謝物

     3. 三級代謝物

三、高分子化合物 (生物聚合物或生物可分解塑膠) 之發酵生產 (黃)

     1. 同聚合物與異聚合物

     2. 培養特性

     3. 構造、性質與功能間之關係

     4. 近代生物技術之應用

四、低分子化合物 (香料或香精組成分) 之發酵生產 (黃)

     1. 香氣化合物之特性

     2. 生產方法

     3. 技術性問題

     4. 評估方法

五、酵母菌之生理與遺傳 (劉)

     1. 酵母菌細胞壁及細胞膜構造與功能

     2. 酵母菌染色體構造與功能

     3. 酵母菌之接合                            

     4. 酵母菌之基因構造        

     5. 酵母菌轉錄作用之調控

     6. GAL 基因

     7. PHO 基因

六、酵母菌之異種蛋白生產 (劉)

     1. 轉譯作用之調控

     2. 傳訊 RNA

     3. 核糖體構造與合成

     4. 胜肽分子合成之起始、延長及終止作用 (劉)

     5. 轉譯後修飾作用

     6. 轉錄與轉譯作用之相關關係

     7. 酵母菌之表現載體

七、酵母菌之蛋白質分泌 (劉)

     1. 蛋白摺疊之分子機制

     2. Chaperone 作用機制

     3. 活性蛋白質之產生

     4. 蛋白質之胞外分泌作用

八、植物細胞培養 (李)

     1. 生合成產物及其應用

     2. 上游培養與下游回收產程之特徵

     3. 應用遺傳工程技術生產生理活性物質與重組蛋白之研究現狀

     4. 展望

 

References

   1) AN Glazer and H Nikaido. 1995. Microbial biotechnology: fundamentals of applied microbiology, W.H. Freeman and Co., New York.

  2) AG Moat, JW Foster and MP Spector. 2002. Microbial Physiology, Wiley-Liss Inc.

  3) S Chattopadhyay, S Farkya, A Srivastava and V Bisaria. 2002. Bioprocess considerations for production of secondary metabolites by plant cell suspension cultures. Biotechnology and Bioprocess Engineering 7:138-149.

  4) TJ Menkhaus, Y Bai, C Zhang, ZL Nikolov and CE Glatz. 2004. Considerations for the recovery of recombinant proteins from plants. Biotechnol Prog 20:1001-1014.

  5) PM Doran. 2000. Foreign protein production in plant tissue cultures. Curr Opin Biotechnol 11:199-204.

  6) R Fischer, E Stoger, S Schillberg, P Christou and RM Twyman. 2004. Plant-based production of biopharmaceuticals. Curr Opin Plant Biol 7:152-158.

Remarks

Lecture in English?  No

B47 M0240

Plant secondary metabolism  植物之二級代謝

Instructor

Ping-Du Lee  李平篤

Outline

Plant Secondary Metabolism is designed for graduate students who are major in the fields of Plant (or Crops)-biochemistry, physiology, or molecular biology. This course covers most of the basic knowledge of Plant Secondary Metabolites, including biochemistry and metabolical regulation for Phenolic Compounds, Terpenoids, Alkaloids, Plant Growth Regulators and Stress-responsed Compounds

Textbook

  1) Buchanan, B.B., Gruissem, W. and Jones, R. L. (2000) Biochemistry and Molecular Biology of Plants. American Society of Plant  Biologists, Maryland, USA.

  2) Lea, P. J. and Leegood, R. C. (1999) Plant Biochemistry and Molecular Biology. 2nd ed. John Wiley & Sons, Chichester, UK.

Remarks

Lecture in English?  No

B47 D0130

Research tools in nutrition  營養學研究法

Instructors

黃青真 (Ching-jang Huang)、蕭寧馨、林璧鳳、潘文涵、呂紹俊

Outline

  1) 營養研究之範疇、層次與基本概念 Scope and basic concept of Nutritional Research

  2) 實驗性營養研究: 由巨觀至微觀 (I) Experimental Type of Nutrition research: Macro to Micro (I)

  3) 實驗性營養研究 (II): 由巨觀至微觀 Experimental Type of Nutrition research: Macro to Micro (II)

  4) 礦物質分析原理與方法

  5) 礦物質營養素代謝平衡原理與評估方法\

  6) 礦物質生體可用率評估原理與方法

  7) 葉酸代謝途徑研究法

  8) 營養免疫功能評估法:細胞培養模式

  9) 營養免疫功能評估法:動物餵食模式

10) 各種營養流性病學研究型態之介紹

11) 流行病學參數在營養學研究上的應用

12) 流行病學分析方法及推論方法在營養學上的應用

13) 膽固醇代謝的研究

14) 分子生物學應用於營養學研究

Textbook  
Remarks

Lecture in English?  No

Course ID

Course title  (credits)

Instructors

 

Outline

 

Textbook

 

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2006/08/04   Juang RH