1. Reading and discuss the articles in “Nature Biotechnology”

2. Methods for microbial cell cultivation and biotransformation (II)

3. The production and evaluation of fermentative functional foods

一、Nature Biotechnology 最新文章之導讀與討論

二、微生物培養與生物轉化方法 (下)

三、機能性發酵食品之生產與評估

Part 1: Searching of data and writing of manuscript

Part 2: Oral presentation and poster presentation

Part 3: Current status of microbial processing

Part 4: Biofunction of proteolytic Enzymes

 I. Introduction (Huang)

II. Fermentative production of bacterial metabolites (Huang)

     1. Primary metabolites

     2. Secondary metabolites

     3. Tertiary metabolites

III. Fermentative production of high molecular weight compounds, biopolymer and biodegradable plastics (Huang)

     1. Homopolymer and heteropolymer

     2. Characteristics of cultivation

     3. Structure-Property-Function relationship

     4. Application of modern biotechnology

IV. Fermentative production of low molecular weight compounds, flavor and fragrance ingredients (Huang)

     1. Characteristics of aroma

     2. Production methods

     3. Technical consideration

     4. Evaluation methods

V. Physiology and genetics of yeast (Liou)

     1. Cell wall and cell membrane

     2. Chromosome structure and function

     3. Mating of yeast                             

     4. Gene structure        

     5. Regulation of transcription

     6. GAL genes

     7. PHO Genes

VI. Heterologous protein expression in microorganism (Liou)

     1. Regulation of translation

     2. Tranfer RNA

     3. Ribosome structure and synthesis

     4. Initiatiion, elongation and termination of polypeptide synthesis

     5. Post-translational processing

     6. Couple of transcription and translation

     7. Expression vector of yeast

VII. Protein excretion in yeast (Liou)

     1. Protein folding stage

     2. Chaperone mechanism

     3. Quality control

     4. Secretion of proteins across the outer mbrane

VIII. Plant cell cultures (Lee)

     1. Products and application

     2. Process characteristics

     3. Molecular approach

一、課程介紹 (黃)

二、細菌代謝物之發酵生產 (黃)

     1. 初級代謝物

     2. 次級代謝物

     3. 三級代謝物

三、高分子化合物 (生物聚合物或生物可分解塑膠) 之發酵生產 (黃)

     1. 同聚合物與異聚合物

     2. 培養特性

     3. 構造、性質與功能間之關係

     4. 近代生物技術之應用

四、低分子化合物 (香料或香精組成分) 之發酵生產 (黃)

     1. 香氣化合物之特性

     2. 生產方法

     3. 技術性問題

     4. 評估方法

五、酵母菌之生理與遺傳 (劉)

     1. 酵母菌細胞壁及細胞膜構造與功能

     2. 酵母菌染色體構造與功能

     3. 酵母菌之接合                            

     4. 酵母菌之基因構造        

     5. 酵母菌轉錄作用之調控

     6. GAL 基因

     7. PHO 基因

六、酵母菌之異種蛋白生產 (劉)

     1. 轉譯作用之調控

     2. 傳訊 RNA

     3. 核糖體構造與合成

     4. 胜肽分子合成之起始、延長及終止作用 (劉)

     5. 轉譯後修飾作用

     6. 轉錄與轉譯作用之相關關係

     7. 酵母菌之表現載體

七、酵母菌之蛋白質分泌 (劉)

     1. 蛋白摺疊之分子機制

     2. Chaperone 作用機制

     3. 活性蛋白質之產生

     4. 蛋白質之胞外分泌作用

八、植物細胞培養 (李)

     1. 生合成產物及其應用

     2. 上游培養與下游回收產程之特徵

     3. 應用遺傳工程技術生產生理活性物質與重組蛋白之研究現狀

     4. 展望

  2) AG Moat, JW Foster and MP Spector. 2002. Microbial Physiology, Wiley-Liss Inc.

  3) S Chattopadhyay, S Farkya, A Srivastava and V Bisaria. 2002. Bioprocess considerations for production of secondary metabolites by plant cell suspension cultures. Biotechnology and Bioprocess Engineering 7:138-149.

  4) TJ Menkhaus, Y Bai, C Zhang, ZL Nikolov and CE Glatz. 2004. Considerations for the recovery of recombinant proteins from plants. Biotechnol Prog 20:1001-1014.

  5) PM Doran. 2000. Foreign protein production in plant tissue cultures. Curr Opin Biotechnol 11:199-204.