Assist. Prof. Lee, KT

Associate Professor

Education．Experience．Service．Teaching．Research．Publication．Results

Kung-Ta Lee Room 117, Building No. 3, NTU
Tel	3366-4436
E-mail	ktlee@ntu.edu.tw
Web
Expertise	Plant cell culture	Applied microbiology	Industrial scale purification of medicinal proteins

Education
Doctor	The University of Tokyo	Department of Biotechnology	1995/04 ~ 1998/03
Master	National Taiwan University	Inst Agric Chem	1989/09 ~ 1991/06
Bachelor	National Taiwan University	Dept Agric Chem	1985/09 ~ 1989/06

EXperience
Dept Biochem Sci Tech (BST)	Associate Professor	Teaching & research	2007/08 ~ now
Dept Biochem Sci Tech (BST)	Assistant Professor	Teaching & research	2003/08 ~ 2007/07
Institute Microbiol Biochem (mbc)	Associate Professor	Teaching & research	2007/08 ~ now
Institute Microbiol Biochem (mbc)	Assistant Professor	Teaching & research	2003/08 ~ 2007/07
Dept Agric Chem	Assistant Professor	Teaching & research	2000/08 ~ 2003/07
Development Center for Biotechnology	Research Fellow	Research	1998/10 ~ 2000/08
The University of Tokyo	Postdoctoral researcher	Research	1998/04 ~ 1998/09
Taiwan Polyamine Co.	Research Fellow	R&D	1991/07 ~ 1995/01

Service
Agricultural Chemical Society of Taiwan	CEO	2003/07 ~ now
Taiwan Association for lactic acid bacteria	Director	2003/07 ~ now
Taiwan Bio Industry Organization	Editor	2002/07 ~ now
Saxophone club of NTU	Advisor	2001/09 ~ now

Current Teaching Courses

Course Title	Credit	Loading	Period
Plant Cell Cultures and Industrial Application	2	1/1	2001/08 ~ now
Applied Microbiology	2	1/4	2001/02 ~ now
Experiment of Applied Microbiology	2	1/2	2001/02 ~ now
Fermentation	2	1/2	2001/09 ~ now

Courses for BST Courses for mbc

Current Research Project

Project Title	Resource	Period
The formation mechanism of hairy root
The biosynthesis route and regulation of podophyllotoxin
Study and application of microbial delignification of lignocellulose	NSC	2009/12 ~ 2010/12
Functional exploration of rol genes in hairy root	NSC	2010/8 ~ 2013/7

Publication 1998~2010

Referred Paper

Lee KT, Yamakawa T, Kodama Y and Shimomura K (1998) Effect of chemicals on alkaloid production by belladonna transformed roots. Phytochemistry 49, 2343-2347.

Lee KT, Yamakawa T, Kodama T, Igarashi Y and Shimomura K (1998) Effects of aeration on tropane alkaloid production by transformed root of Atropa belladonna in flask cultures. J. Ferment. Bioeng. 86, 614-616.

Lee KT, Suzuki T, Yamakawa T, Kodama T, Igarashi Y and Shimomura K (1999) Production of tropane alkaloids by transformed root cultures of Atropa belladonna in stirred bioreactors with a stainless steel net. Plant Cell Rep. 18, 567-571.

Lee KT, Hirano T, Yamakawa T, Kodama T, Igarashi Y and Shimomura K (2001) Responses of transformed root culture of Atropa belladonna to salicylic acid. J Biosci. Bioeng. 91:586-589.

Chen CY, Lee KT, Liu TH, and Liu WH (2004) Callus Induction of Podophyllum pleianthum Hance and the detection of podophyllotoxin. Taiwanese J Agricultural and Food Science 42: 412-420.

Kuo LC, Cheng WY, Wu RY, Huang CJ, Lee KT(2006) Hydrolysis of black soybean isoflavone glycosides by Bacillus subtilis natto. Appl Microbiol Biotechnol. 73:314-320.

Chen SC, Liu HW, Lee KT, Takashi Yamakawa (2007) High efficiency Agrobacterium rhizogenes-mediated transformation of heat inducible sHSP18.2-GUS in Nicotiana tabacum. Plant Cell Rep. 26:29-37

Lee KT, Chen SC, Chiang BL, Yamakawa T (2007) Heat-inducible production of β-glucuronidase in tobacco hairy root cultures. Appl Microbiol Biotechnol. 73:1047-1053.

Kuo, LC; Lee, KT. Cloning, expression, and characterization of two beta-glucosidases from isoflavone glycoside-hydrolyzing Bacillus subtilis natto. (2008) J. Agric. Food Chem. 56:119-125.

Hsu RL, Lee KT, Wang JH, Lee YL, Chen PY (2009) Amyloid-degrading ability of nattokinase from Bacillus substilis natto. J. Agric. Food Chem. 57:503-508.